Synergism in Larvicidal Activity between Supernatant and Pellet from Bacillus thuringiensis Culture
نویسندگان
چکیده
منابع مشابه
Mosquito larvicidal activity of transgenic Anabaena PCC 7120 expressing toxin genes from Bacillus thuringiensis subsp. israelensis.
Genes encoding the mosquito larvicidal toxins Cry4Aa, Cry11Aa, Cyt1Aa and the regulatory P20 from Bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing, filamentous cyanobacterium Anabaena PCC 7120 for expression under control of two strong promoters P(psbA) and P(A1). The clone pRVE4-ADRC displayed toxicity against fourth-instar larvae of Aedes aegypti, the highest...
متن کاملStability of the larvicidal activity of Bacillus thuringiensis subsp. israelensis: amino acid modification and denaturants.
The Bacillus thuringiensis subsp. israelensis mosquito larvicidal toxin is not a sulfhydryl-activated toxin. The protein disulfide bonds were cleaved and blocked without loss of toxicity. In contrast, modification of the lysine side chains eliminated toxicity. Additionally, the toxin was resistant to high concentrations of salt (8 M NaBr), organic solvents (40% methanol), denaturants (4 M urea)...
متن کاملSynergism between Bacillus thuringiensis Spores and Toxins against Resistant and Susceptible Diamondback Moths (Plutella xylostella).
We studied the effects of combinations of Bacillus thuringiensis spores and toxins on the mortality of diamondback moth (Plutella xylostella) larvae in leaf residue bioassays. Spores of B. thuringiensis subsp. kurstaki increased the toxicity of crystals of B. thuringiensis subsp. kurstaki to both resistant and susceptible larvae. For B. thuringiensis subsp. kurstaki, resistance ratios were 1,20...
متن کاملToxicity and synergism in transgenic Escherichia coli expressing four genes from Bacillus thuringiensis subsp. israelensis.
The genes cyt1Aa and p20, encoding, respectively, cytolytic and accessory proteins of Bacillus thuringiensis subsp. israelensis, were introduced into previously constructed clones expressing cry4Aa and cry11Aa in Escherichia coli (Ben-Dov et al., 1995). Fifteen clones with all possible combinations of the four genes were obtained and found to express the genes included. Two new combinations, pV...
متن کاملLarvicidal Activity of Bacillus laterosporus Against Mosquitoes
In present study Bacillus laterosporus isolated from a commercial formulation showed larvicidal activity against second instar larvae of Aedies aegypti, Anophles stephense and Culex qunquefasciatus. The % mortality without heat treatment was 37.34 against Anophles and 18.67 against Culex. The toxin was heat stable and its toxicity increased with inoculum size and incubation time. The study reve...
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ژورنال
عنوان ژورنال: Applied Entomology and Zoology
سال: 1995
ISSN: 0003-6862,1347-605X
DOI: 10.1303/aez.30.153